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杨青课题组

杨青课题组

Yang Qing Lab

  课题组长

  杨青,中国农业科学院(深圳)农业基因组研究所、中国农业科学院植物保护研究所、大连理工大学教授。国家杰出青年基金获得者。长期从事绿色农药分子靶标研究,针对病原菌、有害昆虫等均以几丁质作为结构成分的特点,运用结构生物学和化学生物学等方法系统研究这些生物的几丁质合成、降解以及修饰的关键酶,开发新的、人/畜安全的农药分子靶标以及农化产品。任中国生物化学与分子生物学学会理事,中国昆虫学会昆虫生物化学与分子生物学专业委员会副主任等职务。先后主持国家杰出青年基金、国家自然科学基金重点项目和国家重点研发计划项目子课题等。发表SCI论文100余篇。以通讯作者在ACS Catalysis, Biotechnology Advances, Journal of Medicinal Chemistry, Journal of Biological Chemistry和Journal of Agriculture and Food Chemistry等领域内重要刊物发表多篇论文,出版英文专著1部。

  

  工作经历

  2019.12-至今         中国农业科学院(深圳)农业基因组研究所      研究员         

  2019.5-2019.12     中国农业科学院植物保护研究所                       研究员                                 

  2006.5-2007.12     加州大学伯克利分校                                        访问学者

   2005.7-2006.4      哈佛大学                                                         博士后        

  2007.1-2019.5       大连理工大学                                                  教授

  2003.6-2007.1       大连理工大学                                                  副教授

  1995.7-2003.7       大连理工大学                                                  讲师 

                                                                                                                            

  教育经历

  大连理工大学                   博士         1998.3-2002.3

  大连理工大学                   硕士         1992.9-1995.7

  华东理工大学                   学士         1988.9-1992.7

  

  团队研究方向

  本课题组研究方向包括:I.昆虫表皮及外骨骼形成的分子机制;II.农业有害生物关键蛋白的结构生物学及抑制剂设计;III.病虫害的分子调控

  2020年课题组承担课题包括:国家自然科学基金重点项目1项、国家自然科学基金青年基金项目2项,博士后科学基金2项。发表论文10篇,其中影响因子大于10文章2篇。出站博士后2名。

  

  研究进展

  课题组利用高速原子力显微镜研究了昆虫GH18家族几丁质酶混合物在结晶几丁质表面水解过程中影响其进程性的关键因素。

  在单分子水平上,我们发现内切性几丁质酶OfChtI和OfChtII有助于提升外切酶OfChi-h在结晶几丁质水解中的进程性能力,显著增加每个几丁质酶新结合到几丁质表面积单位上的OfChi-h的数量,增加OfChi-h分子在几丁质表面上进程性运行的持续时间。我们发现当有内切性几丁质酶存在时,OfChi-h分子聚集在几丁质纤丝的末端并形成簇,迅速消融几丁质纤维丝。OfChi-h对底物的结合与络合影响了其进程性水解。根据OfChi-h分子在几丁质原纤维上的排列行为,我们推断出进行性启动的时间为0.22±0.07 s。这项工作提高了我们在结晶几丁质水解的固液界面催化过程中对内切几丁质酶协同作用的认识,突出了内切几丁质酶在促进进行性水解中的作用。该项工作发表于ACS Catalysis.

 

  PI

  Qing Yang, Principle Investigator, Doctoral Advisor, is a professor at the Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, and Dalian University of Technology. She is a recipient of The National Science Fund for Distinguished Young Scholars. Professor Yang has been engaged in the research of molecular targets of green pesticides for a long time. He has been studying the key enzymes of chitin synthesis, degradation and modification of chitin in pathogenic bacteria and harmful insects using structural biology and chemical biology, and exploring new human/animal-safe pesticide molecular targets and developing agrochemical products.

  Professor Yang is the director of the Chinese Society of Biochemistry and Molecular Biology and the deputy director of the Professional Committee of Insect Biochemistry and Molecular Biology of the Chinese Entomological Society. She has presided over the key projects of The National Science Fund for Distinguished Young Scholars, National Natural Science Foundation of China and the sub-projects of National Key Research and Development Program. Professor Yang has published more than 100 SCI papers, including corresponding authorship in ACS Catalysis, Biotechnology Advances, Journal of Medicinal Chemistry, Journal of Biological Chemistry and Journal of Agriculture and Food Chemistry, etc. She has also published one monograph in English.

 

  Working Experience

  2019.12–Present       Agricultural Genomics Institute at Shenzhen-CAAS                                              Professor                                                                             

  2019.5–2019.12        The Institute of Plant Protection, Chinese Academy of Agricultural Sciences       Professor      

  2006.5–2007.12        University of California, Berkeley                                                                           Visiting scholar                                                         

  2005.7-2006.4           Harvard University                                                                                                   Postdoctoral research fellow     

  2007.1-2019.5           Dalian University of Technology                                                                             Professor     

  2003.6-2007.1           Dalian University of Technology                                                                             Associate Professor        

   1995.7-2003.7          Dalian University of Technology                                                                          Lecturer    

  

  Education

  1998.3-2002.3      Dalian University of Technology                                 Ph.D            

  1992.9-1995.7      Dalian University of Technology                                  Master         

  1988.9-1992.7      East China University of Science and Technology       Bachelor      

 

  Research Interest

  Our research fields include: I. Molecular mechanism of insect epidermis and exoskeleton formation; II. Structural biology study and inhibitor design of key proteins of agricultural pests development; III. Molecular regulation of agricultural pests and plant diseases.

  The research projects undertaken by our group in 2020 include: 1 NSFC key project, 2 NSFC Youth Foundations, and 2 Postdoctoral Science Foundations. We published 10 papers in 2020, including 2 papers with impact factors greater than 10. Two postdocs finished their research work.

 

  Major Achievements

  We employed HS-AFM to investigate the factors that affect processivity using an insect GH18 chitinase cocktail during the interfacial enzymatic hydrolysis of crystalline chitin.

  At the single molecule level, we have revealed endochitinases (OfChtI and OfChtII) as a significant factor to elevate the processivity of OfChi-h in crystalline chitin hydrolysis, to dramatically increase the number of OfChi-h newly bound onto a chitin surface area unit per chitinase concentration, and to increase the duration time of processive running OfChi-h molecules on the chitin surface. In the presence of endochitinases, OfChi-h molecules were observed to crowd at the ends of chitin fibrils and form clusters that quickly ablate chitin fibrils. The processive initiation including initial OfChi-h-substrate binding and complexation is a factor that affects processive hydrolysis. The time for processive initiation was deduced to be 0.22 ± 0.07 s according to an aligning behavior of OfChi-h molecules on chitin fibrils. This work improves our knowledge of exo−endo chitinase synergism during the solid−liquid interfacial catalysis of crystalline chitin hydrolysis, highlighting the role of endochitinases in boosting processive hydrolysis. This work was published in ACS Catalysis.

 

  Selected Publication

  主编专著书籍

  1.   Yang Q*, Fukamizo T*. Targeting Chitin-containing Organisms. Advances in Experimental Medicine and Biology. Springer Nature. 2019

  发表的代表性论著

  1.  Mingbo Qu, Takahiro Watanabe-Nakayama, Shaopeng Sun, Kenichi Umeda, Xiaoxi Guo, Yuansheng Liu, Toshio Ando*, Qing Yang.* 2020. High-speed atomic force microscopy reveals factors affecting the processivity of chitinases during interfacial enzymatic hydrolysis of crystalline chitin. ACS catalysis, 10: 13606-13615.

  2.  Wei Chen, Xi Jiang, Qing Yang.⁎ 2020. Glycoside hydrolase family 18 chitinases: The known and the unknown. Biotechnology Advances, 43: 107553.

  3.  Xi Jiang,# Ashutosh Kumar,# Yasutaka Motomura, Tian Liu, Yong Zhou, Kazuyo Moro, Kam Y. J. Zhang,* Qing Yang.* 2020. A series of compounds bearing a dipyrido-pyrimidine scaffold acting as novel human and insect pest chitinase inhibitors. Journal of Medicinal Chemistry, 63: 987−1001.

  4.  Wei Chen, Qing Yang*. 2020. Development of novel pesticides targeting insect chitinases: A minireview and perspective. Journal of Agricultural and Food Chemistry, 68: 4559−4565.

  5.  Xiaoming Zhao, Jing Zhang, Jiapeng Yang, Niu Niu, Jianzhen Zhang,∗ Qing Yang∗. 2020. Mucin family genes are essential for the growth and development of the migratory locust, Locusta migratoria. Insect Biochemistry and Molecular Biology, 123: 103404.

  6.  Tian Liu, Xiaoguang Guo, Yunfei Bu, Yong Zhou, Yanwei Duan, Qing Yang*. Structural and biochemical insights into an insect gut-specific chitinase with antifungal activity. Insect Biochemistry and Molecular Biology. 2020,119:103326.

  7.  Huitang Qi, Tian Liu*, Qiong Lu, Qing Yang. Molecular insights into the insensitivity of lepidopteran pests to cycloxaprid. Journal of Agricultural and Food Chemistry.2020,68,982-988.

  8.  Dong L, Shen S, Chen W, Lu H, Xu D, Jin S, Yang Q*, Zhang J *. Glycosyl triazoles as novel insect β-N-acetylhexosaminidase OfHex1 inhibitors: Design, synthesis, molecular docking and MD simulations. BIOORGANIC & MEDICINAL CHEMISTRY, 2019, 27: 2315-2322. [IF 2.79, Q2]

  9.  Chen W, Zhou Y, Yang Q *. Structural dissection reveals a general mechanistic principle for group II chitinase (ChtII) inhibition. JOURNAL OF BIOLOGICAL CHEMISTRY, 2019, 294: 9358-9364.

  10.  Shen S, Dong L, Chen W, Wu R, Lu H, Yang Q *, Zhang J*. Synthesis, Optimization, and Evaluation of Glycosylated Naphthalimide Derivatives as Efficient and Selective Insect β- N-Acetylhexosaminidase OfHex1 Inhibitors. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2019, 67: 6387-6396.

  11.  Dong Y, Hu S, Jiang X, Liu T, Ling Y, He X, Yang Q *, Zhang L *. Pocket-based Lead Optimization Strategy for the Design and Synthesis of Chitinase Inhibitors. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2019, 67: 3575-3582.

  12.  Chu F#, Wang D#, Liu T, Han H, Yu Y, Yang Q *. An optimized cocktail of chitinolytic enzymes to produce N,N’-diacetylchitobiose and N-acetyl-D-glucosamine from defatted krill by-products. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2019, 133: 1029-1034.

  13. Yang H#, Qi H#, Liu T, Shao X, Yang Q *, Qian X*. Naphthalimide and quinoline derivatives as inhibitors for insect N-acetyl-β-D-hexosaminidase. CHINESE CHEMICAL LETTERS. 2019, 30(5): 977-980.

  14. Yang J, Bai J, Qu M, Xie B, Yang Q*. Biochemical characteristics of a nitroreductase with diverse substrate specificity from Streptomyces mirabilis DUT001. BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY. 2019, 66(1): 33-42.

  15. Liu L, Zhou Y, Qu M, Qiu Y, Guo X, Zhang Y, Liu T, Yang J, Yang Q*. Structural and biochemical insights into the catalytic mechanisms of two insect chitin deacetylases of the carbohydrate esterase 4 family. JOURNAL OF BIOLOGICAL CHEMISTRY. 2019,294(15):5774-5783.

  16. Xi Jiang, Han Bao, Hans Merzendorfer, Qing Yang*. Immune Responses of Mammals and Plants to Chitin-Containing Pathogens. ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY. 2019,1142: 61-81.

  17. Qing Yang*, Tamo Fukamizo*. An Introduction to the Book. ADVANCES IN EXPERIMENTAL MEDICINE AND BIOLOGY. 2019,1142:1-4.

 

  Selected Patent

  1. 杨青;赵小明,飞蝗粘蛋白Mucin17、其编码基因与应用,2019.2.25,中国,201910136232.2

  2. 杨青;刘田;韩鸿宇;王迪,AnChi基因、AnChi几丁质酶及其在降解几丁质方面的应用,2018.01.18,中国,201810048548.1

  3. 杨青;姜熙;刘田;杨君;屈明博,一种几丁质酶抑制剂及其应用,2018.05.9, 201810439288.0

  4. 杨青;屈明博;刘田;杨君;陈磊;刘元盛,一种利用昆虫几丁质酶降解固体几丁质生产N-乙酰氨基葡萄糖的方法,2018.1.17,中国,201810044270.0

  5. 杨青;刘田;王迪;韩鸿宇;李安节,一种细菌来源几丁质酶及其在制备GlcNAc中的应用,2018.01.24,中国,201810069069.8 

  6.杨青; 刘田; 褚方萌; 韩鸿宇,组合酶法降解脱脂南极磷虾粉回收多肽中间产物及制备N-乙酰葡萄糖胺的方法,2018.4.24, CN201810373432.5

  7. 杨青;刘田;姜熙;杨君;屈明博, 春雷霉素及其衍生物为几丁质酶抑制剂的应用, 2017.04.24,中国,201710271627.4

  8. 杨青;邓晓瑞;屈明博;杨君;刘田,昆虫I型几丁质结合蛋白AA10基因及其在害虫防治中的应用,2017.11.30,中国,201711237537.X

  9. 杨青;刘田;段燕伟;唐静仪;屈明博;杨君,小檗碱及其衍生物作为己糖胺酶抑制剂的应用,2017.03.31,中国,201710216474.3 

  10. 杨青;刘田;王迪;陈磊;卜云飞,高效几丁质酶突变体的制备方法及应用,2016.10.19,中国,201610910629.9

  11. 杨青;屈明博;邓晓瑞;陈威,一种昆虫来源的多结构域内切几丁质酶;其相关的生物材料及其应用,2016.09.06,中国,201610803226.4

  12. 杨青;刘田;陈磊;段燕伟;路新华,一种抑制剂及其在抑制几丁质酶和己糖胺酶活性中的应用,2016.12.16,中国,2016111705166

  13. 杨君;白敬;刘培瑜;刘田;姜秀萍;杨青,一种高活性细菌硝基还原酶基因及其应用,2014.07.15,中国,201410337143.1

  14. 杨君;夏悦昕;白敬;屈明博;刘田;姜秀萍;杨青,一种鳞翅目昆虫二氢叶酸还原酶的基因、其干扰基因及其应用,2014.04.25,中国,01410172992.6

  15. 杨君; 白敬; 刘培瑜; 姜熙; 杨青,一种区位选择性细菌硝基还原酶基因及其应用,2014.10.15,中国,CN201410336949.9

  16. 杨君; 白敬; 刘培瑜; 姜熙; 杨青,一种区位选择性细菌硝基还原酶基因、其重组酶及其应用,2014.10.15, 中国, CN201410337184.0

  17. 杨青;杜昱光;刘田;赵勇;陈磊,一种作为几丁质酶抑制剂的3-7聚葡萄糖胺单体,2014.01.10,中国,201410013553.0

  18. 杨青; 杜昱光; 刘田; 赵勇; 赵小明; 杨君; 姜秀萍,壳寡糖作为昆虫几丁质酶抑制剂及杀虫剂的应用,2013.1.10, 中国, 201310010126.2

  19. 杨青; 钱旭红; 刘田; 郭朋,一种萘酰亚胺衍生物作为酶抑制剂、杀虫剂,2014.4.16,中国,201310539176.X

  20. 郭朋;刘田;陈琦;徐林;杨青;钱旭红,萘酰亚胺衍生物及其用途,2012.11.01, 中国,201210430633.7

  21. 杨青; 姜秀萍; 贾秋磊; 杨君; 刘田,绿盲蝽多聚半乳糖醛酸酶的基因及其应用,2012.7.18, 中国,201110407055

  22. 杨青;周罡;杨君;姜秀萍;刘田,亚洲玉米螟β-N-氨基乙酰葡萄糖基水解酶OfOGA基因及其应用,2011.12.23,中国,201110440659.5

  23. 杨青; 谢波; 杨君; 陶黎明; 钱旭红,一种高效还原芳香酮的链霉菌及其在生物催化中的应用,2009.6.24, 中国,200810013512.6

  24. 杨青; 屈明博; 刘田; 杨君,利用几丁质生产能源物质的方法,2007, 中国,2007101130799

  

  杨青课题组更新于2021年2月

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