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Zuo Erwei Lab


  左二伟,研究员,课题组组长。2015年获得广西大学动物繁殖学博士,2015年至2018年,中国科学院神经科学研究所非人灵长类研究组从事博后工作。长期从事动物遗传育种繁殖和基因编辑技术的相关研究。以第一作者身份在国内外高水平期刊Science、Cell Research、Genome biology等发表文章。

  Erwei Zuo, Principal Investigator. He obtained his Ph.D. from Guangxi University, where he studied the animal reproduction science. His postdoctoral training was at the Institute of Neuroscience (ION) of Chinese Academy of Sciences (CAS),where he was dedicated to animal genetic breeding and gene editing related research. He has became an independent investigator at the Agricultural Genome Institute at Shenzhen, Chinese Academy of Agricultural Sciences since 2018. His lab aims to explore genome editing and other potential applications for CRISPR-Cas systems.




  Gene editing methods and technology in mammalian animals. (1) Improving reproductive efficiency of genetic engineering pigs by using stem cell. (2) Optimization of gene editing technology, including broadening the application scope, improving specificity, and reducing off-target effect. (3) Construction of animal disease models in mice and pigs by genomic manipulation.




  We developed a method named “GOTI”, and found that cytosine base editor induced substantial off-target single nucleotide variants (SNVs). GOTI significantly improved the sensitivity of off-target detection without prediction in advance and could also detect randomly generated off-target variants. Thus, GOTI has provided as a new breathtaking method for the safety evaluation of genome editing tools, which could be taken as industry standard.



1. Yuan,T.*, Yan,N.*, Fei,T.*, Zheng,J.*, Meng,J.*, Li,N.*, Liu,J.*, Zhang,H., Xie,L., Ying,W., Li,D., Shi,L., Sun,Y.,Li,Y., Li,Y., Sun,Y.# and Zuo,E.#.(2021). Optimization of C-to-G base editors with sequence context preference predictable by machine learning methods.Nature Communications: 12:4902

2. Xue,Y.*, Hu,X.*,Wang,D.*, Li,D.*,Li,Y.,Wang,F.,Huang,M., Gu,X., Xu,Z., Zhou,J., Wang,J., Chai,R., Shen,J., Chen,Z., Li,G., Yang,H., Li,H.#, Zuo,E.# and Shu,Y.#. (2021).Gene editing in a Myo6 semi-dominant mouse model rescues auditory function Author links open overlay panel .Molecular Therapy. DOI: 10.1016/j.ymthe.2021.06.015

3. Zhang,J.*, E,M.Khazalwa.*, H, M.Abkallo., Zhou,Y., Nie,X., Ruan,J., Zhao,C., Wang,J., Xu,J., Li.X., Zhao,S., Zuo,E.#, L,Steinaa#.and Xie,S.#.(2021).The Advancements, Challenges and Future Implications of the CRISPR/Cas9 System in Swine Research.Journal of Genetics and Genomics:48(5).347-360.
4. Wei,Y.*, Zhou, Y.*, Liu,Y.*, Ying,W., Lv,R., Zhao,Q.,  Zhou,H., Zuo,E.#,  Sun,Y.#, Yang,H.# and Zhou,C,#. (2021). Indiscriminate ssDNA cleavage activity of CRISPR-Cas12a induces no detectable off-target effects in mouse embryos.Protein & Cell . DOI: 10.1007/s13238-021-00824-z

5. Huang, X.*, Lv, J.,*, Li, Y.*, Mao, S., Li, Z., Jing, Z., Sun, Y., Zhang,X., Shen, S., Wang, X., Di, M., Ge, J., Huang, X., Zuo,E.# and Chi, T.#.(2020). Programmable C-to-U RNA editing using the human APOBEC3A deaminase .The EMBO Journal:39:e104741.

6. Zuo,E.*, Sun,Y. *, Wei,Wu.*, Yuan ,T.*, Ying,W., Sun,H., Yuan,L., Lars M. Steinmetz # , Li,Y.# and Yang,H.#.(2020).GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos.Natrue Protocols:15.3009–3029.

7. Lee,S.*, Ding,N.*, Sun,Y., Yuan,T., Li,J., Yuan,Q., Liu,L.,Yang,J.,Wang,Q., Anatoly B. Kolomeisky, Isaac B. Hilton, Zuo,E.# and Gao,X.#. (2020).Single C-to-T substitution using engineered  APOBEC3G-nCas9 base editors with minimum  genome- and transcriptome-wide off-target effects.Science Advance:29(6).1773.

8. Zuo,E.*#, Sun,Y.*, Yuan ,T.* , He,B.* , Zhou,C.* , Ying,W., Liu,L., Wei ,W., Zeng,R., Li,Y.# and Yang,H.#.(2020).A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.Nature Methods:17(6):600–604.

9. Lin X.*, Chen H.*, Lu Y.*, Hong S.*, Hu X.*, Gao Y., Lai L., Li J., Wang Z., Ying W., Ma L., Wang N., Zuo,E.#, Yang H.# and Chen W. #.(2020).Base editing-mediated splicing correction therapy for spinal muscular atrophy. Cell Research:0:1-3. 30(6).

10. Deng K.*, Feng W.*, Liu X.*, Su X., Zuo,E., Du S., Huang Y., Shi D. # and Lu,F. # .(2020).Anti-silencing Factor 1A is Associated with Genome Stability Maintenance of Mouse Preimplantation Embryos.Biology of Reproduction:102(4),817-827.

11. Li J. *, Lin X. *, Tang C. *, Lu Y. *, Hu X. *, Zuo,E., Li H., Ying W., Sun Y. , Lai L., Chen H., Guo X., Zhang Q., Wu S., Zhou C., Shen X., Wang Q., Lin ., Ma L., Wang N.,Ad.Krainer, Shi L.#, Yang H.# and Chen W.#.(2020) .Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice.National Science Review:7(1),92-101.

12. Zhao, X., Wei, W., Pan,H., Nie,J., Chen,D., Zhang,P., Chen, F., Fu, Q,. Zuo, E.#, Lu, Y.# and Zhang, M.#. (2019). Identification of the Sex of Pre-implantation Mouse Embryos Using a Marked Y Chromosome and CRISPR/Cas9. SCIENTIFIC REPORTS:9: 14315.

13. Li,J.* , Hong,S.*, Chen,W.#, Zuo,E.# and Hui Yang#.(2019) . Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing.Journal of Genetics and Genomics:46,513-521.

14. Wang, X.*, Kang, J.*, Wei, L.*, Yang, X.*, Sun, H., Yang, S., Lu, L., Yang, M., Bai, M., Chen, Y., Long, J., Li, N., Li, D., Huang, J., Lei, M., Shao, Z., Yuan, W.#, Zuo, E.#, Lu, K. #, Liu, M.# and Li, J#. (2019). PHF7 is a novel histone H2A E3 ligase prior to histone-to-protamine exchange during spermiogenesis. Development: dev.175547.

15. Zhou, C.*, Sun, Y.*, Yan, R.*, Liu, Y.*, Zuo, E.*, Gu, C., Han, L., Wei, Y., Hu, X., Zeng, R., Li, Y.#, Zhou, H.#, Guo, F.# and Yang, H.# .(2019).Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis. Nature:571, 275–278.

16. Zuo, E.*, Sun, Y.*, Wei, W.*, Yuan, T.*, Ying, W., Sun, H., Yuan, L., Steinmetz, L. M.#, Li, Y.# and Yang, H#.(2019). Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science:364, 289-292.

17. Zuo, E.*, Huo, X.*, Yao, X.*, Hu, X.*, Sun, Y.*, Yin, J*., He, B., Wang, X., Shi, L., Ping, J., Wei, Y., Ying, W., Wei, W., Liu, W., Tang, C., Li, Y., Hu, J.# and Yang, H.#. (2017).CRISPR/Cas9-mediated targeted chromosome elimination. Genome Biology:18:224.

18. Zuo, E.*, Cai, Y. J.*, Li, K.*, Wei, Y.*, Wang, B. A.*, Sun, Y.*, Liu, Z., Liu, J., Hu, X., Wei, W., Huo, X., Shi, L., Tang, C., Liang, D., Wang, Y., Nie, Y. H., Zhang, C. C., Yao, X., Wang, X., Zhou, C., Ying, W., Wang, Q., Chen, R. C., Shen, Q., Xu, G. L., Li, J., Sun, Q., Xiong#, Z. Q.# and Yang, H#.(2017).One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs. Cell Research:27, 933-945.

19. Wang,L. *, Li,M. *, Qu, C.,Miao,W., Yin,Q., Liao,J., Cao,H., Huang,M., Wang,K. Zuo,E. , Peng,G., Zhang,S., Chen,G., Li,Q., Tang,K., Yu, Q., Li,Z., Catherine CL Wong, Xu,G., Jing, N.,Yu,X.,# and Li,J.#.(2017).CRISPR-Cas9-mediated genome editing in one blastomere of two-cell embryos reveals a novel Tet3 function in regulating neocortical development.Cell Research:1-15.

20. Zuo, E. *, Yang, X.#, Lu, Y., Xie, L., Shang, J., Li, D., Yang, H., Hu, L., Zhao, H., Lu, S., Lu, K.#.(2015). ZPAC is required for normal spermatogenesis in mice. Mol. Reprod:Dev. 82: 747-755.

21. Zhang, M.*, Zhou, H., Zheng, C. ,Xiao, J. , Zuo, E., Liu, W., Xie, D.,Shi, Y.,Wu, C., Wang, H. , Li, D. & Li, J .#.(2014).The Roles of Testicular C-kit Positive Cells in De novo Morphogenesis of Testis. SCIENTIFIC REPORTS:4 : 5936.

22. Liu,H.*, Lv,P., Zhu,X., Wang,X., Yang,X., Zuo,E., Lu,Y., Lu, S., Lu,K.#.(2014).In vitro development of porcine transgenic nuclear-transferred embryos derived from newborn Guangxi Bama mini-pig kidney fibroblasts.In Vitro Cell.Dev.Biol.—Animal:50:811–821.