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费启立课题组

来源: 发布者: 时间:2020年02月13日

  费启立课题组 

  Fei Qili Lab

  课题组长

  费启立,研究员,博导。2016年博士毕业于美国特拉华大学,研究方向为植物小分子RNA。2016-2019年在美国芝加哥大学从事表观转录组学研究,尤其是RNA甲基化修饰N6-methyladenosine (m6A)。迄今在PLOS Biology, PNAS, Plant Cell,Plant Journal,Plant Biotechnology Journal,Journal of Experimental Botany等杂志发表论文十余篇。

 

  工作经历

  2019.9–至今               中国农业科学院深圳农业基因组研究所      研究员                                                                             

  2016.9–2019.8           芝加哥大学                                          博士后                                                                              

  

  教育经历

  2012.1–2016.8                 特拉华大学                      博士                   

  2012.1–2015.12               特拉华大学                      硕士                   

  2007.9–2011.6                 西北农林科技大学            学士                   

  

  研究方向

  真核生物转录组的RNA修饰,尤其是甲基化修饰N6-methyladenosine (m6A),对转录本RNA的剪接、降解、翻译等过程起着重要的调控作用。课题组将以植物生长发育、生物及非生物胁迫等生物学问题为背景,利用生物化学、分子生物学、遗传学,以及高通量测序等技术手段,深入研究转录组表观修饰的生物学功能。同时,实验室还开展小分子RNA的组学和功能研究,探究其在植物基因表达调控以及物种演化中的意义。

 

  研究进展

  真核生物小分子RNA以及转录组RNA甲基化修饰m6A对基因的转录后水平调控起着重要的作用。费启立及其合作者在原来研究miRNA介导产生phasiRNA机制的基础上(Plant Cell, 25(7): 2400; Plant Journal, 83(3): 451),发现了菜豆族植物中的特有的miRNA的前体二级结构会抑制miRNA末端甲基化修饰(2’-O-methylation)并促进其尿苷化作用(PNAS, 115(31):8037),从而产生22-nt的miR1510并介导产生phasiRNA。不同于2’-O-methylation, m6A修饰更广泛存在于转录组RNA上,近期的研究揭示了m6A结合蛋白YTHDF2在人类细胞中通过促进转录本RNA的降解参与细胞周期调控的分子机制(PLOS Biology, accepted)。

 

  PI

  Qili Fei, Principle Investigator, Doctoral Advisor . He mainly focuses on plant small RNAs and RNA modifications, including N6-methyladenosine (m6A). He has published more than 10 papers in journals such as PLOS Biology, PNAS, Plant Cell, Plant Journal, Plant Biotechnology Journal, Journal of Experimental Botany.

  

  Working Experience

  2019.9–Present               Agricultural Genomics Institute at Shenzhen-CAAS            Professor                                                                         

  2016.9–2019.8               The University of Chicago                                                     Postdoctoral Associate            

 

  Education Experience

  2012.1–2016.8               University of Delaware                                         Ph.D                   

  2012.1–2015.12             University of Delaware                                         M.S.                   

  2007.9–2011.6               Northwest A&F University                                   B.S.        

            

  Research Interest

  Eukaryotic transcriptomic modifications (epitranscriptome), especially N6-methyladenosine (m6A), play important roles in splicing, degradation, and translation. Our lab will study the roles of epitransciptomic modifications in plant development and biotic/abiotic stresses. We aim to dissect the roles of RNA modifications through different approaches, such as biochemistry, molecular biology, genetics, as well as high through-put sequencing. At the same time, we will study the roles of small RNAs in plant gene expression regulation and evolution.

 

  Major Achievements

  Small RNAs and RNA modifications, such as m6A, play important roles in gene regulation at the post-transcriptional level in eukaryotes. Based on our previous studies on the mechanisms of phasiRNA biogenesis triggered by miRNAs (Plant Cell, 25(7): 2400; Plant Journal, 83(3): 451), we have demonstrated that the secondary structure of miR1510/miR1510* duplex may inhibit the terminal 2’-O-methylation activity of HEN1, thus leading to the production of 22-nt miR1510 by uridylation. Different from 2’-O-methylation, m6A is more broadly present on mRNAs. We recently revealed that the m6A reader protein YTHDF2 plays important roles in cell cycle regulation by promoting the mRNA turnover in human cells.

 

  Selected Publication

  1. Fei, Q.*, Zou, Z.*, Roundtree, I.A., Sun, H.L., and C. He. (2020) YTHDF2 promotes mitotic entry and is regulated by cell cycle mediators. PLOS Biology (accepted). (*co-first authors)

  2. Bi, H.*, Fei, Q.*, Li, R., Liu, B., Xia, R., Char, S.N., Meyers, B.C., and B. Yang. (2019) Disruption of miRNA sequences by TALENs and CRISPR/Cas9 induces varied lengths of miRNA production. Plant Biotechnology Journal. doi: 10.1111/pbi.13315. (*co-first authors)

  3. Fei, Q., Yu, Y., Liu, L., Zhang, Y., Baldrich, P., Dai, Q., Chen, X., and B.C. Meyers. (2018) Biogenesis of a 22-nt microRNA in Phaseoleae species by precursor-programmed uridylation. Proceedings of the National Academy of Sciences of the United States of America. 115(31):8037-8042.

  4. Fei, Q.*, Yang, L.*, Liang, W., Zhang, D., and B.C. Meyers. (2016) Dynamic changes of small RNAs in rice spikelet development reveal specialized reproductive phasiRNA pathways. Journal of Experimental Botany. 67(21):6037-6049. (*co-first authors)

  5. Fei, Q., Zhang, Y., Xia, R., B.C. Meyers. (2016) Small RNAs add zing to the Zig-Zag-Zig model of plant defenses. Molecular Plant-Microbe Interactions. 29(3): 165-169.

  6. Fei, Q., Li, P., Teng, C., B.C. Meyers. (2015) Secondary siRNAs from Medicago NB-LRRs modulated via miRNA-target interactions and their abundances. The Plant Journal. 83(3): 451-65.

  7. Fei, Q., Xia, R., and B.C. Meyers. (2013) Phased, secondary, small interfering RNAs in posttranscriptional regulatory networks. The Plant Cell. 25(7): 2400–2415.

  8. Xu, H., Dzhashiashvili, Y., Shah, A., Kunjamma, R.B., Weng, Y.L., Elbaz, B., Fei, Q., Jones, J.S., Li, Y.I., Zhuang, X., Ming, G.L., He, C., and B. Popko. (2020) m6A mRNA Methylation Is Essential for Oligodendrocyte Maturation and CNS Myelination. Neuron. 105(2):293-309.e5.

  9. Hsu, P.J., Fei, Q., Dai, Q., Shi, H., Dominissini, D., Ma, L., and C. He. (2019) Single base resolution mapping of 2’-O-methylation sites in human RNA and in 3’ terminal ends of small RNAs. Methods. pii: S1046-2023(18)30194-4.

  10. Wei, J., Liu, F., Lu, Z., Fei, Q., Ai, Y., He, P.C., Shi, H., Cui, X., Su. R., Klungland, A., Jia, G., Chen, J., and C. He. (2018) Differential m6A, m6Am, and m1A demethylation mediated by FTO in the cell nucleus and cytoplasm. Molecular Cell. 71(6):973-985.

  11. Liu, H., Soyars, C., Li, J., Fei, Q., He, G., Peterson, B., Meyers, B.C., Nimchuk, Z.L., and X. Wang. (2018) CRISPR/Cas9‐mediated resistance to cauliflower mosaic virus. Plant Direct. 2 (3), e00047.

  12. Shu, X., Dai, Q., Wu, T., Bothwell, I.R., Yue, Y., Zhang, Z., Cao, J., Fei, Q., Luo, M., He, C., and J. Liu. (2017) N6-Allyladenosine: a new small molecule for RNA labeling identified by mutation assay. Journal of the American Chemical Society. 139(48):17213-17216.

  13. Yang, L., Qian, X., Chen, M., Fei, Q., Meyers, B.C., Liang, W., and D. Zhang. (2016) Regulatory role of a receptor-like kinase in specifying anther cell identity in rice. Plant Physiology. 171(3):2085-100.

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