Principal Investigator

Xinjie Wang Lab

【字体：大中小】


Xinjie Wang
Researcher
Ph.D., Northwest A&F University, 2017
Email: wangxinjie@caas.cn
Introduction
My research focuses on developing biosensors for the detection of pathogen infections in livestock and microbial contaminants during food production and farming. During the past three years, we have developed a series of rapid and sensitive CRISPR-based detection methods for viruses, bacteria, and gene mutations. With the advanced technologies, we will continue to develop novel biosensors for the detection of the biological contaminants in the food and agricultural systems, such as infectious pathogens, specific DNA, toxins, allergens, etc. We are always looking for enthusiastic assistant researchers, postdocs, and graduate and undergraduate students to work with us. Please contact Dr. Wang (wangxinjie@caas.cn) if you are interested.
Research Interest
Fast and accurate nucleic acid detection is the foundation of precision treatment, farming safety, and food safety. The ideal detection technology should be sensitive, specific, fast, cost-effective, and requires no equipment. Detection schemes, including PCR-based methods, isothermal amplification, and next-generation sequencing, still have various limitations. My research focuses on developing the next-generation CRISPR-based diagnostic biosensors, which will contribute to the detection of pathogens, toxins, and allergens in animal husbandry and food. With the technologies of synthetic biology, nanomaterials, microfluidics, and artificial intelligence, we aim to develop novel detection technology that is more sensitive and specific, faster, easier to use, and more intelligent.
Selected Publications
1.	Liu Y, Chen YL, Dang L, Liu YX, Huang SS, Wu SY, Ma PX, Jiang HQ, Li YY, Pan YB, Wei YC, Ma XD, Liu M, Ji QJ, Chi T, Huang XX#, Wang XJ#, Zhou FL#. 2021. EasyCatch, a convenient, sensitive and specific CRISPR detection system for cancer gene mutations. Molecular Cancer 20, 157.
2.	Ma PX, Meng QZ, Sun B, Zhao B, Dang L, Zhong MT, Liu SY, Xu H, Mei H, Liu J, Chi T, Yang G, Liu M#, Huang XX#, Wang XJ#. 2020. MeCas12a, a Highly Sensitive and Specific System for COVID‐19 Detection. Advanced Science doi:10.1002/advs.202001300.
3.	Wang XJ, Liu ZW, Li GL*, Dang L, Huang SS, He L, Ma YE, Li C, Liu M, Yang G, Huang XX, Zhou F, Ma XD. 2020. Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation. Molecular Therapy 28:431-440.
4.	Wang XJ, Zhong MT, Liu Y*, Ma PX, Dang L, Meng QZ, Wan WW, Ma XD, Liu J, Yang G, Yang ZF, Huang XX, Liu M. 2020. Rapid sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER. Science Bulletin 65:1436-1439.
5.	Wang XJ, Ji PP, Fan HY, Dang L, Wan WW, Liu SY, Li YH, Yu WX, Li XY, Ma XD, Ma X, Zhao Q, Huang XX, Liao M. 2020. CRISPR/Cas12a technology combined with immunochromatographic strips for portable detection of African swine fever virus. Communications Biology 3:62.
6.	Wang XJ*, Shang XY, Huang XX. 2020. Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods. Emerging Microbes & Infections 9:1682-1691.
7.	Liu Y., Chen Y., Huang S., Ma X., Huang X., Wang XJ#., Zhou F#. 2021. Rapid and Sensitive Diagnosis of Drug-Resistant FLT3-F691L Mutation by CRISPR Detection. Frontiers in Molecular Biosciences 8.
8.	Meng Q., Yang H., Zhang G., Sun W., Ma P., Liu X., Dang L., Li G., Huang X., Wang XJ#., Liu J#., Leng Q#. 2021. CRISPR/Cas12a-assisted rapid identification of key beer spoilage bacteria. Innovative Food Science & Emerging Technologies 74.
9.	Meng QZ, Wang XJ, Wang YQ, Dang L, Liu XY, Ma XD, Chi T, Wang X, Zhao Q, Yang G, Liu M, Huang XX, Ma PX. 2021. Detection of the SARS‐CoV‐2 D614G mutation using engineered Cas12a guide RNA. 2021. Biotechnology Journal. (Cover story)
10.	Wang XJ, Zhao Q, Dang L, Sun YN, Gao JM, Liu BY, Syed SF, Tao H, Zhang GP, Luo JX, Zhou EM. 2015. Characterization of Two Novel Linear B-Cell Epitopes in the Capsid Protein of Avian Hepatitis E Virus That Are Common to Avian, Swine, and Human HEVs. Journal of Virology 89.
11.	Wang XJ, Zhao Q, Jiang FL, Liu BY, Zhao JN, Dang L, Sun YN, Mu Y, Xiao SQ, Wang CB, Hsu WH, Liu LH, Widen F, Zhou EM. 2014. Genetic characterization and serological prevalence of swine hepatitis E virus in Shandong province, China. Veterinary Microbiology 172:415-424.
12.	Dang L, Li GL, Wang XJ, Huang SS, Zhang Y, Miao YX, Zeng L, Cui SZ, Huang XX. 2020. Comparison of gene disruption induced by cytosine base editing-mediated iSTOP with CRISPR/Cas9-mediated frameshift. Cell Proliferation 53:e12820.
13.	Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang XJ, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MKH, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG. 2019. Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. Elife 8.

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Xinjie Wang Lab