Introduction

　　Fanghao Wan, a Research professor and doctoral advisor, Chief scientist and consultant expert at the National Program on Key Basic Research Project (973 Program), member of Expert National Biosecurity Key Project. His principal research interest is biological invasion and biological control. He created the emerging subject of invasion biology, in China. He successively presided over by 973 program and other Major Program more than 20 so on. He won 20 national and provincial achievement awards (among which two were second prize of national science and technology progress awards). He has published more than 700 papers (SCI more than 160) and 21 monographs.

　　Working Experience

　　2014.10–Present　　  Agricultural Genomics Institute at Shenzhen-CAAS                                                      Research professor                 

        2006.3–2014.12           Institute of Plant Protection-CAAS                                                                                 Research professor   

　　2004.7–2006.3             Institute of Environment and Sustainable Development in Agriculture -CAAS            Research professor   

　　1995.9–2004.7             Institute of Biological Control -CAAS                                                                           Research professor          

　　1993.9–1995.9            Agriculture and Agri-Food Canada, Research Station, Lethbridge, AB.                        Postdoctoral                                                                      

　　1989.9–1993.9            Institute of Biological Control -CAAS                                                                           Associate professor 

　　1984.12–1986.12        Hunan Agricultural College                                                                                            Research assistant    

　　Education Experience

　　1986.12–1989.9                     Chinese Academy of Agricultural Sciences          Ph.D                   

        1982.1–1984.12                     College of Hunan agricultural                               Master               

　　1978.3–1982.1                       College of Hunan agricultural                                Bachelor         

　　Research Interest

　　Our team mainly focus on the genomics study of critical invasive alien species in agriculture, aimed at obtaining an in-depth understanding of the invasion mechanism as well as developing novel techniques for control and management based on whole genome sequencing and deep mining of omics big data. Firstly, we build high-quality genome assemblies for invasive alien species using state-of-the-art genome sequencing technique and assembly strategy, and elucidate the molecular mechanism and ecological process of invasion combining with biological experiments. Additionally, the important genes related to invasiveness are discovered. Furthermore, molecular insecticide or herbicide targeting those genes and intelligent detection and monitoring system are developed, achieving innovation of techniques for control and management of invasive species.

　　Major Achievements

　　1. The chromosome-level genome illuminates the molecular mechanism of chemical-ecological adaption and insecticide resistance in Cydia pomonella

　　We sequenced and assembled the chromosome-level genome of Cydia pomonella. Comparative genomics analysis showed that the OR3 gene was duplicated, which enhanced the adaptation ability to hosts in C. pomonella during the global invasion. The CYP6B2 gene was identified by Genome-wide association study (GWAS), which associated with the insecticide resistance of C. pomonella. Our results provide a new view and method for the comprehensive control of C. pomonella.

　　Fig. 1 Functional demonstration of CpomOR3a/CpomOR3b in pear ester and codlemone olfactory reception of the codling moth, Cydia pomonella.

　　2. Identification and developmental expression of juvenile hormone esterase (CpJHE) in codling moth, Cydia pomonella (L.)

　　We identified the juvenile hormone esterase gene (CpJHE) of Cydia pomonella and found that the CpJHE transcript level take a leap in the 3-day-old fifth instar larva, and it expressed mainly in the fat body. This study indicates that the CpJHE gene play a vital role in the development and reproduction of C. pomonella and it can provide a new target for the prevention and management of C. pomonella.

　　Fig. 2 Identification and development expression of juvenile hormone esterase gene (CpJHE) in Cydia pomonella

　　3.Genome sequencing of invasive mollusk and discovery of invasion related genes

　　The latest sequencing techniques and assembly strategies were employed to build a chromosome-scale genome of Achatina immaculata. Meanwhile, we performed comparative genomic analysis, obtaining important genes in relation to invasiveness and conducting functional validation. Additionally, we performed whole genome re-sequencing analysis of different populations. In total, this study provides insights into the molecular mechanism for the successful invasion of Giant African snails in genome scale.

　　Fig. 3. The general characteristics of A.immaculata. Track n: 31 linkage groups of the genome; Track d-m: expression profile of brain, egg, eye, hemocytes, hepatopancreas, kidney, lungs, muscle, ovary and testis tissues; Track c: distribution of transposon elements; Track b: distribution of gene density; Track a: distribution of GC content.

　　4. Genome sequencing of Mikania micrantha and discovery of invasion related genes

　　Using high-throughput sequencing and multiple genomic techniques, the research team systematically analyzed the molecular mechanism of environmental adaptive evolution and rapid growth during the invasion of Mikania micrantha from the perspectives of family expansion of important genes in the genome, allelochemical synthesis pathway, strong photosynthetic system and nitrogen cycle in rhizosphere soil.

　　Fig. 4 Molecular mechanism of rapid growth in Mikania micrantha; a, Evolution of compositae; b, Whole-genome replication events in compositae; c, sesquiterpene lactone metabolism pathway of Mikania micrantha; D. CO2 fixation mode of Mikania micrantha during day and night; Nitrogen cycling in the rhizosphere of Mikania micrantha.

　　5. Invasive alien plants intelligent monitoring

　　We acquired the color images of the monitoring area in wild environment by unmanned aerial vehicle (UAV), and propose a novel IAPsNet based on deep convolutional neural network (CNN) to identify the seven invasive alien plants appeared in the images, the recognition accuracy was 93.39%. And IAPsNet has successfully applied to intelligently monitor the IAPs in the real-time. The research results will provide some theoretical and scientific basis for the development of intelligent monitoring and early warning system of invasive alien plants.

　　Fig. 5 invasive alien plants images acquired by UAV, and deep CNN architecture of IAPsNet

　　6. IAS1000 Project

　　The “IAS1000 Project” (A genome project of 1000 invasive alien species,) has been successfully carried out. At present, it has established cooperative relations with more than 40 scientific research institutes, universities and enterprises. The first list important invasive species, such as Mikania micrantha, Eupatorium adenophorum, Cydia pomonella, Tuta sp.., Spodoptera exigua, Eriosoma lanigerum, Lissorhoptrus oryzophilus, Pomacea canaliculata and Achatina immaculata, were sequenced and analyzed.

　　Notes: “★” represents different population re-sequencing.

　　Selected Publication

　　1. Bo Liu, …, Wanqiang Qian*, Wei Fan* and Fanghao Wan*. (2020) Mikania micrantha genome provides insights into the molecular mechanism of rapid growth. Nature Communications, DOI: 10.1038/s41467-019-13926-4. （SCI收录）

　　2. Fanghao Wan #*, Chuanlin Yin#, Rui Tang#, Maohua Chen#, Qiang Wu#, Cong Huang#, Wanqiang Qian, …, Fei Li*. A chromosome level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance. Nature Communications, 2019, 10: 4237. （SCI收录）

　　3. Cong Huang, Qiang Wu, Chunyan Jiang, Longsheng Xing, Guoliang Shi, Bin Zhang, Wanqiang Qian, Youzhi Li, Yu Xi, Nianwan Yang, Fanghao Wan*. Identification and developmental expression of putative gene encoding juvenile hormone esterase (CpJHE-like) in codling moth, Cydia pomonella (L.). Journal of Integrative Agriculture, 2019, 18(7): 1624-1633. （SCI收录）

　　4. Longsheng Xing, Tianbo Ding, Cong Huang, Yu Xi, Qiang Wu, Wanqiang Qian, Fanghao Wan, Bin Zhang*. 2019. A comprehensive atlas of lysine acetylome in onion thrips (Thrips tabaci Lind.) revealed by proteomics analysis. Journal of Proteomics, 207 (2019) 103465. （SCI收录）

　　5. Bin Zhang, Wanqiang Qian, Xi Qiao, Yu Xi, Fanghao Wan. 2019. Invasion biology, ecology, and management of Frankliniella occidentalis in China. Archives of Insect Biochemistry and Physiology,  DOI:10.1002/arch.21613. （SCI收录）

　　6. Fanghao Wan. 2019. Plant protection: Current status, progress and challenges in China. Journal of Integrative Agriculture, 18(4): 703-704. （SCI收录）

　　7. Xi Qiao, Jianhua Bao, Hang Zhang, Fanghao Wan, Daoliang Li. 2019. Underwater sea cucumber identification based on principal component analysis and support vector machine. Measurement, 133:444-455. （SCI收录）

　　8. Xiong zhang#, Bo Liu#,…, Wei Fan*, Wanqiang Qian*, Daolong Dou*. Whole Genome Re-sequencing Reveals Natural Variation and Adaptive Evolution of Phytophthora sojae. Frontiers in Microbiogy, 2019, doi: 10.3389/fmicb.2019.02792.（SCI收录）

　　9. 黄聪, 李有志, 杨念婉, 武强, 邢龙生, 钱万强, 席羽, 李飞, 万方浩*. 入侵昆虫基因组研究进展. 植物保护, 2019, 45(5): 112-120.

　　10. 张雪，钱万强，王宁，周倩，张彬*. 不同光照条件对西花蓟马产卵量的影响. 中国植保导刊，2019, 39(7): 5-10.

　　11. 鲁莎，郭建洋，席羽，万方浩*. miRNA调控昆虫与病害互作的研究进展.生物安全学报，2019,28(2):89-94.

　　Selected Copyrights

　　1、基因染色体定位图绘制软件V1.0,登记号：2019SR0544532,中国农业科学院深圳农业基因组研究所（邢龙生）

　　2、入侵植物识别系统V1.0,登记号：2019SR0544523,中国农业科学院深圳农业基因组研究所（乔曦）