1. Established a "one-step" technology system to generate complete knockout animals and effectively shorten the time of producing genetic animals.

2. Established a whole chromosome knockout technique that offers a new approach to developing animal models with chromosome deletions, and a potential therapeutic strategy for human aneuploidy diseases involving additional chromosomes.

3. Developed “GOTI”, a novel off-target detection technology that can detect completely random off-target which is undetectable by previous means. Moreover, this strategy significantly improved the sensitivity of off-target detection for gene editing technology, brought a breakthrough new tool for safety assessment of gene editing tools, and potentially became a new industry standard for detection.

4. Designed a new version of high-fidelity gene editing technology that allows for high-efficiency and high-fidelity C-G single-base editing, effectively reducing off-target efficiency.