In recent years, the lab has been committed to the research and development and application of new gene editing technologies by means of molecular biology and computational biology and promoted the construction of a safety evaluation system for the medical application of gene editing technology. The molecular basis of off-target effects is proposed, and a variety of detection technologies are developed to confirm that single-base editors can lead to completely random and unpredictable off-target effects in the genome; the relationship between the nucleic acid binding domain of deaminase and off-target effects is clarified, and single-base editing tools with good efficiency and precision are developed, which solves the major problems of medical application to a certain extent; the treatment of mouse models of a variety of human diseases has been realized, and good results have been obtained, which provides a basis for clinical experiments and is expected to treat related diseases. Among them, a large number of off-target effects caused by single-base gene editing and their optimization solutions were selected as "Top Ten Advances in Life Sciences in China in 2019", and the development of high-precision single-base editing tools and new off-target detection technologies was selected as "Major Advances in Chinese Medicine in 2019". Rationally designed cytosine base editor can reduce the non-targeting effects of DNA and RNA while maintaining high targeting activity, and was selected as the "2020 Major Scientific Discovery" of the Chinese Academy of Agricultural Sciences and won the "Major Achievement Award" of the 2021 Genome Research Institute. The single-base editor off-target detection technology and high-fidelity base editor development won the "2022 Youth Science and Technology Innovation Award of the Chinese Academy of Agricultural Sciences". The one-step production of gene complete knockout animals won the "Merit Award" at the 2017 International Society for Stem Cell Research Annual Meeting (2017 ISSCR). In recent years, he has published a number of research papers on gene editing technology in Science, Nature and other journals.