1.Development of special nucleic acid structure profiling techniques: There are various special nucleic acid structures in organisms, such as R-loop and DNA breaks, which play important roles in development and stress response. The reported R-loop and DNA break detection techniques have many deficiencies in resolution, sensitivity, efficiency, etc., which limit the development of related research fields. Based on the R-loop sequencing technology ssDRIP-seq and DNA damage sequencing technology DEtail-seq, the group is developing ultra-low-input, high-throughput, universal special nucleic acid profiling techniques based on high-efficiency single-stranded DNA ligation technology to further improve the sensitivity, applicability and accuracy of the techniques.

2.High-throughput epigenome sequencing technology development: The research fields of epigenetics mainly include DNA modification, histone modification, RNA modification, non-coding RNA and so on. Epigenetic mechanisms play important roles on the regulation of gene expression in crops. However, the reported epigenomics technologies have many disadvantages including low throughput, high cost, and low stability, which makes the epigenetic data output in the field of agriculture insufficient, seriously limits the researches in agricultural epigenetics and related fields. To address the above problems, novel high-throughput epigenetic sequencing technologies will be developed by the following means: 1) High-throughput DNA modification sequencing technology through the development of pre-barcoding technology, combined with antibodies targeting specific DNA modifications, such as 6mA and 5mC; 2) Upgrading the traditional ChIP-seq by pre-barcoding methods, and establishing a high-throughput mChIP-seq method, geometrically increasing the library construction throughput. 3) Developing a high-throughput mRIP-seq technology based on the RNA pre-barcoding technology combined with anti-RNA modification antibodies.